Thyroid-stimulating hormone (thyrotropin) is a glycoprotein released from the anterior pituitary. The synthesis and secretion of TSH is stimulated by thyrotropin releasing hormone (TRH), a tripeptide produced by the hypothalamus. TSH stimulates the thyroid gland to produce thyroxine (T4) and triiodothyronine (T3) by binding to the TSH receptor. The circulating free fractions of T4 and T3 in turn regulate the secretion of TSH by a negative feedback mechanism at the pituitary and the hypothalamus.

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