4 ML (2 ML MIN.) PLASMA FROM 2 LAVENDER TOP (EDTA) TUBES. OVERNIGHT FASTING IS MANDATORY. COLLECT SPECIMEN WITH PATIENT IN SUPINE POSITION AFTER MINIMUM 30 MINUTES OF REST. IMMERSE SPECIMEN IMMEDIATELY IN ICE WATER. SEPARATE PLASMA WITHIN 30 MINUTES AND TRANSFER INTO STERILE SCREW CAPPED VIAL. SHIP REFRIGERATED OR FROZEN. PATIENT SHOULD STRICTLY AVOID DRUGS LIKE LABETALOL, SOTALOL, ACETAMINOPHEN, METHYLDOPA, ANTIDEPRESSANTS AND AGENTS LIKE EPHEDRINE, AMPHETAMINE, NICOTINE AND CAFFEINE ETC. AVOID FOODS LIKE BANANA, PINEAPPLE, NUTS AND CEREALS ETC. AT LEAST 72 HOURS PRIOR TO SAMPLE COLLECTION.