3 ML WHOLE BLOOD IN 1 BLUE TOP (SODIUM CITRATE) TUBE. MIX THOROUGHLY BY INVERSION. TRANSPORT TO LAB WITHIN 4 HOURS. IF THIS IS NOT POSSIBLE, MAKE PPP WITHIN 1 HOUR OF COLLECTION AS FOLLOWS: CENTRIFUGE SAMPLE AT 3600 RPM FOR 15 MIN. & TRANSFER SUPERNATANT TO A CLEAN PLASTIC TUBE. CENTRIFUGE THIS SUPERNATANT AGAIN AT 3600 RPM FOR 15 MIN. & FINALLY TRANSFER THE SUPERNATANT (PPP) TO 1 LABELLED CLEAN PLASTIC SCREW CAPPED VIAL. FREEZE IMMEDIATELY. SHIP FROZEN. OVERNIGHT FASTING IS MANDATORY. DULY FILLED COAGULATION REQUISITION FORM AND TEST SEND OUT CONSENT FORM IS MANDATORY.