5 mL (4 mL min.) Serum from 2 SSTs. Ship refrigerated or frozen AND 2 mL (1 mL min.) Plasma from 1 Lavender Top (EDTA) tube. Separate plasma within 2 hours of collection. Ship refrigerated or frozen. Patient should avoid Biotin at least 8 hours prior to specimen collection AND 3 mL (2 mL min.) Whole blood from 1 Lavender Top (EDTA) tube. Ship refrigerated. DO NOT FREEZE AND 3 mL Whole blood in 1 Blue Top (Sodium Citrate) tube. Mix thoroughly by inversion. Transport to Lab within 4 hours. If this is not possible, make PPP within 1 hour of collection as follows: Centrifuge sample at 3600 rpm for 15 min. & transfer supernatant to a clean plastic tube. Centrifuge this supernatant again at 3600 rpm for 15 min. & finally transfer the supernatant (PPP) to one labelled clean plastic screw capped vial. FREEZE IMMEDIATELY. Ship frozen. Overnight fasting is preferred. Duly filled .