This assay is used for differentiating absorptive from non absorptive causes of hypercalciuria. Therapy for hypercalciuria depends upon the cause. Hypercalciuria due to absorptive causes usually responds to dietary restriction whereas hypercalciuria from other mechanisms usually responds to Thiazides.

10 mL (5 mL min.) aliquot of 2 hour urine collection in a sterile screw capped container. Follow detailed instructions as below: 1) No food or drink after 6 pm a day prior to the test. 2) On the day of the test do not void urine after 8 am. 3) At 10 am void urine and discard. 4) Drink a glass of water at 10 am and 11 am. Drink only deionized distilled water. 5) Collect urine voided at 12 noon in the container with 6N HCl as the preservative. Ship refrigerated or frozen. Record 2 hour volume on the urine vial and test request form.

Arsenazo III

Daily

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Follow detailed instructions as below: 1) No food or drink after 6 pm a day prior to the test. 2) On the day of the test do not void urine after 8 am. 3) At 10 am void urine and discard. 4) Drink a glass of water at 10 am and 11 am. Drink only deionized distilled water.