This assay is useful for investigating possible disorders of mitochondrial metabolism when used in conjunction with blood lactate collected at the same time to determine lactate to pyruvate ratio.
3 mL (2 mL min.) Whole blood from 1 Grey Top (Sodium Fluoride) tube. Mix whole blood immediately with equal volume of 7% Perchloric Acid. Mix well and centrifuge immediately. Separate supernatant and submit minimum 2 mL. Ship refrigerated or frozen.
Spectrophotometry
Sample Daily by 2 pm; Report Same day
This assay is useful for investigating possible disorders of mitochondrial metabolism when used in conjunction with blood lactate collected at the same time to determine lactate to pyruvate ratio.